At least five carriages of a train travelling between Karachi and Lahore came off the rails near the city of Mehrabpur in Sind province overnight.
A policeman at the scene was quoted as saying 50 bodies had been found but there is no official confirmation. Hundreds of people have died in recent years in crashes on Pakistani railways. Correspondents say casualty figures are often so high because trains are packed with far greater numbers than for which they were designed. Signalling faults and poorly maintained tracks are often the cause of the accidents.
More than 130 people were killed when three trains collided near the southern town of Ghotki in July 2005 in the country's worst train disaster for 15 years.
The express train which overturned on Wednesday is said to have been loaded with passengers travelling home for the Islamic festival of Eid al-Adha.
Khalid Amin, a senior railway official, told the Associated Press news agency that two relief trains had been dispatched to pick up survivors.
The cause of the crash was not immediately clear.
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